Cell-cell adhesion proteins

Cadherins are a large family of calcium-dependent cell-cell adhesion molecules that mediate adherens junction formation. In our lab, we study both the activation and the inhibition mechanism of classical cadherins. Our overarching goal is to map the large conformational space associated with the cadherin activation mechanism and to develop selective modulators of cadherin-mediated cell-cell adhesion.

Selected publications

  • Dalle Vedove A, Falchi F, Donini S, Dobric A, Germain S, Di Martino GP, Prosdocimi T, Vettraino C, Torretta A, Cavalli A, Rigot V, Andre’ F, Parisini E.  Structure-based virtual screening allows the identification of efficient modulators of E-cadherin-mediated cell-cell adhesion. Int. J. Mol. Sci., 2019, 20, 3404.
  • Civera M, Vasile F, Potenza D, Colombo C, Parente S, Vettraino C, Prosdocimi T, Parisini E, Belvisi L. Exploring E-cadherin-peptidomimetics interaction using NMR and computational studies. PLOS Comp. Biol., 2019, 15, e1007041.
  • Nardone V, Lucarelli AP, Dalle Vedove A, Fanelli R, Tomassetti A, Belvisi L, Civera M, Parisini E. Crystal structure of human E-cadherin-EC1EC2 in complex with a peptidomimetic competitive inhibitor of cadherin homophilic interaction. J. Med. Chem., 2016, 59, 5089.
  • Doro F, Colombo C, Alberti C, Arosio D, Belvisi L, Casagrande C, Fanelli R, Manzoni L, Parisini E, Piarulli U, Luison E., Figini M., Tomassetti A, Civera M. Computational design of novel peptidomimetic inhibitors of cadherin homophilic interactions. Org. Biomol. Chem., 2015, 13, 2570.
  • Dalle Vedove A, Lucarelli AP, Nardone V, Matino A, Parisini E. The X-ray structure of human P-cadherin EC1-EC2 in a closed conformation provides insight into the type I cadherin dimerization pathway. Acta Crystallogr. Sect F, 2015, F71, 371.
  • Parisini E, Higgins JMG, Liu JH, Brenner MB, Wang JH. The crystal structure of human E-cadherin domains 1 and 2, and comparison with other cadherins in the context of adhesion mechanism. J. Mol. Biol., 2007; 373, 401-411.